Pittsburgh compound B (PiB) is a radioactive analog of thioflavin T, which can be used in positron emission tomography scans to image beta-amyloid plaques in neuronal tissue. Due to this property, Pittsburgh compound B may be used in investigational studies of Alzheimer's disease.
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History of PiB
The definitive diagnosis of Alzheimer's disease can only be made following the demonstration of the presence of beta-amyloid plaques and neurofibrillary tangles, the pathologic hallmarks of Alzheimer's disease in brain tissue, typically at autopsy. While the cognitive impairments of the disease could be monitored throughout the disease course, clinicians had no reliable way to monitor the pathologic progression of the disease. Due to this fact, a clear understanding of the process of amyloid deposition and how amyloid deposits relate to the cognitive symptoms of Alzheimer's disease remains to be elucidated. While sophisticated centers for the treatment of Alzheimer's disease are able to diagnose the disease with some reliability based on its clinical presentation, the differential diagnosis of Alzheimer's disease from other dementias is less robust. Furthermore, as novel disease-modifying therapies for Alzheimer's disease that attack and remove beta-amyloid deposits from the brain enter clinical trials, a pre-mortem tool for assessing their effectiveness at clearing the amyloid deposits was a much needed development.
To answer these needs, a research team from the University of Pittsburgh led by geriatric psychiatrist William E. Klunk and radiochemist Chester A. Mathis synthesised charge neutral benzothiazoles derived from thioflavin T, which included a small number of compounds with suitable properties for use as a positron emission tomography imaging agent. One of these compounds, 2-(4'-[11]methylaminophenyl)-6-hydroxybenzothiazole, was tested in human subjects. The University of Pittsburgh team partnered with a team of researchers from Uppsala University in Uppsala, Sweden, to conduct the first trials of this new agent in human research subjects. As this was the second investigational compound of this class sent to Uppsala from the University of Pittsburgh group, it was termed simply Pittsburgh compound-B by the Swedish team, who also abbreviated it as "PiB".
The first PiB study of a human subject with a clinical diagnosis of Alzheimer's disease was conducted by Henry Engler in February, 2002, at Uppsala University. PET scans showed that the compound was retained in areas of the cerebral cortex known to contain significant amyloid deposits from post-mortem examinations. The initial human study of PiB was expanded to include 16 Alzheimer's disease subjects and 9 cognitively normal controls, the report of which was published in 2004 in the Annals of Neurology.
Since that initial study, PiB has been adopted as a research tool by dozens of research institutions around the world. PiB and other compounds in its class are protected under US 7270800 , issued Sept. 18, 2007, which is presently assigned to the University of Pittsburgh. The technology is under license to GE Healthcare, a subsidiary of General Electric, who is pursuing the development of a clinical diagnostic agent based on PiB for assessing brain amyloidosis.
PIB Role in Alzheimer's Disease Research
11C-PIB is currently the most studied and used radioligand for PET imaging of cerebral Aß pathology. This technique has been implicated in Alzheimer's disease research whereby scientists involved in this field are able to perform noninvasive in vivo neuroimaging studies using PET scans in brains of individuals with various degrees of dementia. The 11C-Pittsburgh Compound B (11C-PIB) radiotracer is used to measure regional 11C-PIB binding retention rates, thus allowing for the visual and quantitative measurement of A? deposition. 11C-PIB is a fluorescent derivative of thioflavin T which preferentially targets and binds to fibrillar A? forms found in dense core plaques with high affinity and specificity. In particular, it specifically binds to Aß40 and Aß42 fibrils and insoluble plaques containing the aforementioned Aß peptides. It should be noted that PIB does not bind with great affinity to soluble or nonfibrillar Aß plaques until plaques have reached a crucial magnitude, which has yet to be determined. Furthermore, this radiotracer does not bind to neurofibrillary tangles (NFTs) in the neuronal regions of the brain during postmortem autopsies. A typical injected dose ranges from 250-450 MBq and the imaging time normally varies between 40 and 90 minutes. The quantification of 11C-PIB has demonstrated to elicit a profound difference in neuronal cortical binding between individuals recognized with Alzheimer's disease and age-matched cognitively normal controls.
Published clinical research studies using PiB
Source of the article : Wikipedia
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